The present study was conducted to develop a toxoplasma latex agglutination test antigen(Test-MT) and evaluate the toxoplasma latex agglutination(LA) test using a newly-made "Test-MT kit" by comparing with the Toxo-MT kit(Eiken chemical co, Tokyo). Also, the specifity and sensitivity test were made by comparing with IFA test and IgG-ELISA.
Tachyzoite suspensions of Toxoplasma gondii(RH strain) were ultracentrifuged for 30min at 60,000×g(4℃) and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of 1.0㎛ in diameter(Polyscience co) were used for the preparation of sensitized latex-antigen supension(Test-MT).
The frequency distribution of LA titers in Test-MT showed two peaks at < 1:32 and 1:128. The borderline titer for positive test in Test-MT was determined to be 1:64. But the frequency distribution of LA tites in Toxo-MT showed two peaks at < 1:16 and 1:64. The positive borderline was determined to be 1:32.
Agreement of reactions between Test-MT and Toxo-MT kit by LA test was shown 92.5% in bovine sera and 97.0% in swine sera, respectively.
From the results obtained here it was determined that the sensitized latex-antigen, Test-MT kit, for the microtiter agglutination test prepared as same as by the procedure described in the previous paper(Suh and Lee, 1993) was useful as a highly specific, sensitive and stable immunotiteration reagent for serodiagnosis of toxoplasrna infection in animal sera.
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